National Centre for Sustainable Coastal Management
Ministry of Environment, Forest and Climate Change
Government of India

Microbiology and Biotechnology Facility

The Microbiology and Biotechnology facility at NCSCM offers a wide range of resources and support that assist scientists and researchers in monitoring and solving marine environmental issues. The major focus area includes:

Coral-Microbe Association

  • Investigation on functional characteristics of coral-microbe dynamics in response to Environmental stresses (eg; temperature, pH, salinity)
  • Reconstruction of broader network between microbial communities and metabolomics of beneficial and pathogenic microbes associated with corals
  • Extraction of novel compounds from commensal bacteria that inhibit coral pathogens

Oil pollution and Hydrocarbonoclastic bacteria

  • Isolation of potential hydrocarbonoclastic bacteria from oil polluted marine environments
  • Functional dynamics of microbial communities and fate of oil in the marine ecosystem
  • Formulation of oil degrading bacterial consortia for bioremediation processes

Inventorisation and database creation on microbial diversity along Indian Coast Consortium

  • Formation of a knowledge base to offer broad spectrum information about the coastal microbes which will provide deeper insights into the microbial diversity, their temporal and spatial variation, and changing profile with respect to climate change

DNA Barcoding

  • Application of taxonomic methods for identification of an unknown/novel marine organism with the pre-existing classification that aid in development of coastal and marine biodiversity integration network

Analysis Type

:
  • Isolation and characterization of microbial cultures
  • 16S rRNA/18S rRNA sequencing and identification
  • Environmental biodiversity screening

Type of Facility

:

Research Facility


Thematic Areas

:
  • Microbial Ecology
  • Microbial Biotechnology
  • Marine Biodiversity

Contact Information

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Lab-in-charges
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Instruments

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  • Agarose-Gel Electrophoresis Unit

    Model : Hoofer-PS300-B

    Agarose-Gel Electrophoresis Unit

    Separation of DNA/RNA based on the molecular weight and charge. Used in checking the quality of DNA/RNA by determining the intensity of the bands for molecular identification of prokaryotes/eukaryotes.

  • Autoclave - Automatic

    Model : Panasonic MLS3781L

    Autoclave - Automatic

    Used in sterilization/decontamination of culture media, microbial cultures, reagents, plasticware and glassware typically at 121 C (250 F), 15psi, for 15-20min.

  • Bacteriological Incubator

    Model : Rays

    Bacteriological Incubator

    Used for growing/enriching microbial cultures by incubating at specific temperatures ranging from 24-45˚C usually for 24-72 hrs.

  • Binocular Stereo-Zoom microscope with camera
    Make : Nikon
    Model : SMZ-25

    Binocular Stereo-Zoom microscope with camera
    For identification and enumeration of phytoplankton, zooplankton and other marine organisms from different ecosystems.

  • Cooling Micro-Centrifuge

    Model : LAB-KITS-CFTGL2M

    Cooling Micro-Centrifuge

    Used for low temperature-high speed centrifugation procedures of molecular analysis such as DNA/RNA/protein extraction, purification and separation.

  • Denaturing Gradient Gel Electrophoretic (DGGE)

    Model : Cipher DTSK

    Denaturing Gradient Gel Electrophoretic (DGGE)
    Separation of DNA based on their melting domain and difference in GC content. Used in detecting the structure of microbial communities in various ecosystems like water, sediment, sewage etc.

  • Epifluorescent Microscope

    Model : Leica DM2500

    Epifluorescent Microscope

    To enumerate and identify differentially fluorescing dead and live microbial cultures/cells present in environmental samples.

  • Flow Cam
    Make : Portable Color Flow
    Model : Camcx VS-IV-C P

    Flow Cam

    To enumerate or analyze particles/microbes/ plankton accurately, reliably and quickly using automated imaging technology.

  • Gel Documentation System

    Model : Gel-Doc-UVP

    Gel Documentation System

    Visualization and documentation of nucleic acid (DNA/RNA) and protein suspended within polyacrylamide or agarose gels after gel electrophoresis

  • Incubator Shaker
    Make : INFORS HT
    Model : Multitron Pro

    Incubator Shaker

    For cell culturing, cell aeration, and solubility studies under controlled RPM, temperature and photic conditions in laboratory.

  • Laminar Airflow Chamber

    Model : Cleanair

    Laminar Airflow Chamber

    Aseptic cabinet for safe handling of microbes and fluids aided by UV sterilization and HEPA filtration.

  • Sonicator

    Model : Sonics

    Sonicator

    Used to disrupt the membranes of the cells/microbial cultures for extraction of compounds/nucleic acids by applying ultra sound (high-frequency energy)

  • Microbial Identification System

    Model : Sherlock™ MIDI - Agilent

    Microbial Identification System

    Identification of microorganisms based on gas chromatographic (GC) analysis of extracted microbial fatty acid methyl esters.

  • Microplate Reader

    Model : Spetramax M2e

    Microplate Reader

    Used to detect chemical, biological or physical reactions by measuring emitted light in terms of Absorbance, florescence or luminescence in samples/microbial cultures.

  • Nanodrop One

    Model : Thermo scientific

    Nanodrop One

    Quantification of Nucleic acid (DNA/RNA/PCR amplicon) and protein based on the difference in absorbance of the molecules at nanogram quantities.

  • Polymerase Chain Reactor (PCR)

    Model : Veriti-Applied biosytems

    Polymerase Chain Reactor (PCR)

    Used in amplification of DNA/RNA (to several thousand folds) for various purposes such as gene detection, identification by sequencing and cloning.

  • SDS - Poly Acrylamide Gel Electrophoresis (PAGE) Unit

    Model : Biorad Mini-protean

    SDS - Poly Acrylamide Gel Electrophoresis (PAGE) Unit
    Used in separation of proteins based on molecular weight and charge. As well as purification and elution of unknown proteins for various molecular analyses.